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KMID : 0379120230510030219
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Korean Journal of Mycology
2023 Volume.51 No. 3 p.219 ~ p.227
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Fungal Distribution in Traditional Meju and Characterization of Isolated Strains
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Son Ye-Eun
Kang Ye-Jin
Choi Sun-Young
Choi Yoon-Kyung
Lee Ju-Eun
Kim Jun-Young
Park Hee-Soo
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Abstract
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This study was conducted to analyze the distribution and characteristics of fungal species in meju using the traditional method. Fungal distribution in meju was investigated using metagenomic and morphological analyses, based on which Aspergillus flavus/oryzae strains were identified as the dominant fungi in all meju samples, followed by Pichia, Rhizopus
and Lichtheimia spp. As A. flavus/oryzae was dominant, we further evaluated the aflatoxin production ability and enzymatic activity of the isolates. Thin-layer chromatography and polymerase chain reaction revealed that the A. flavus/oryzae strains isolated from meju are non-aflatoxigenic fungi. Based on the analyses of amylase and protease activities, strains with high activities of amylase or protease were identified, which are proposed to be used as
starters for meju fermentation.
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KEYWORD
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Aspergillus, Aflatoxins, Enzymatic activity, Meju, Metagenomic analysis
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